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Amino acid sequence determination of the novel forms of G o α purified from bovine brain membranes
Author(s) -
Shibasaki Hisayuki,
Kozasa Tohru,
Takahashi Katsunobu,
Inanobe Atsusi,
Kaziro Yoshito,
Ui Michio,
Katada Toshiaki
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80814-j
Subject(s) - complementary dna , protein subunit , peptide sequence , amino acid , cdna library , elution , chemistry , biology , microbiology and biotechnology , biochemistry , chromatography , gene
Previously we have reported that there are at least four different forms of G o α in bovine brain membranes which can be distinguished by their elution profiles from Mono Q column and their immunological reactivities. The four α‐subunits are referred to as α o 1 , α o 2 , α o 3 and α o 4 in their elution orders from the column. Partial amino acid sequences of the purified α o 1 and α o 2 were determined and compared with the predicted sequences of two classes of G o α cDNAs, termed G o α‐1 and G o α‐2. There were at least two unique fragments corresponding with the predicted amino acid sequence of the G o α‐2 cDNA but different from that of the G o α‐1 cDNA upon tryptic digestion of α o 1 ‐ or α o 2 ‐subunit. The α o 3 ‐ and α o 4 ‐subunits, but not α o 1 ‐ and α o 2 ‐subunits, were recognized by an antibody raised against a unique amino acid sequence predicted from G o α‐1 cDNA. These results suggest that α o 1,2 subunits and α o 3,4 subunits are encoded by G. o α‐2 cDNA and G o α‐1 cDNA, respectively.