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Receptor‐activated cytoplasmic Ca 2+ spikes in communicating clusters of pancreatic acinar cells
Author(s) -
Petersen C.C.H.,
Petersen O.H.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80774-w
Subject(s) - cytoplasm , cholecystokinin , chemistry , biophysics , acetylcholine , patch clamp , cell , receptor , acinus , pancreas , endocrinology , biology , biochemistry
The transmission of receptor‐generated cytoplasmic Ca 2+ signals between communicating pancreatic acinar cells has been investigated by comparing patch‐clamp recordings of Ca 2+ ‐dependent Cl − current in internally perfused single cells and small multi‐cell clusters. Acetylcholine (50 nM) generates shortlasting repetitive spikes of Ca 2+ ‐dependent current and these spikes are not transmitted to neighbouring cells. Cholecystokinin octapeptide (5 pM) also generates repetitive spikes, but a significant proportion of these trigger longer and larger pulses of Ca 2+ ‐dependent current and these waves can easily spread from cell to cell. In pancreatic acinar units it is therefore possible to observe both local Ca 2+ signals confined to the cell of its origin as well as Ca 2+ signals that spread through communicating junctions to all cells in the unit.