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Degradation of cells dying by apoptosis leads to accumulation of ε(γ‐glutamyl)lysine isodipeptide in culture fluid and blood
Author(s) -
Fesus Laszlo,
Tarcsa Edit,
Kedei Noemi,
Autuori Francesco,
Piacentini Mauro
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80773-v
Subject(s) - tissue transglutaminase , apoptosis , lysine , phagocytosis , proteolysis , involution (esoterism) , chemistry , biochemistry , biology , microbiology and biotechnology , enzyme , amino acid , consciousness , neuroscience
ε(γ‐Glutamyl)lysine isodipeptide, the end‐product of proteolytic digestion of proteins cross‐linked by transglutaminase, was detected in culture fluid of neonatal rat hepatocytes and plasma of adult rats. The concentration of the isodipeptide was significantly increased in both when high rate of apoptosis with phagocytosis of dying hepatocytes was produced either by epidermal growth factor in the culture or by lead nitrate‐induced hyperplasia with subsequent involution in rats. Specific induction of tissue transglutaminase and the consequent formation of highly cross‐linked protein envelopes in apoptotic cells have been previously demonstrated by us in both systems.