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Regulation of glutathione S ‐transferase gene expression by phenobarbital in cultured adult rat hepatocytes
Author(s) -
Vandenberghe Yves,
Tee Lisa,
Morel Fabrice,
Rogiers Vera,
Guillouzo André,
Yeoh George
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80772-u
Subject(s) - microbiology and biotechnology , phenobarbital , glutathione , northern blot , messenger rna , gene expression , glutathione s transferase , biology , blot , complementary dna , in vitro , hepatocyte , cell culture , gene , enzyme , biochemistry , endocrinology , genetics
Previous studies, by using Northern blotting analyses, showed that phenobarbital (PB) affects the steady‐state mRNA levels of glutathione S ‐transferase (GST) subunits , and 7 in both conventional cultures of adult rat hepatocytes and co‐cultures, with rat liver epithelial cells [Vandenberghe et al., 1989, FEBS Lett. 251, 59–64; Morel et al., 1989, FEBS Lett. 258, 99–102]. To determine whether PB acts at the transcriptional level, nuclear ‘run‐on’ experiments using cDNA probes hybridizing to GST subunits , and 7 mRNA were performed on purified nuclei isolated from control and PB treated hepatocytes seeded under conventional and co‐culture conditions. Data from this study demonstrate that the increase in steady‐state mRNA levels observed in both conventional culture and co‐culture after 4 days PB exposure results from an increased transcriptional activity or the GST genes. However, a substantial increase in steady‐state mRNA levels in the absence of a commensurate increase in transcriptional activity at 12 days of co‐culture, indicates that the barbiturate has also a stabilizing effect in vitro on the GST mRNAs.