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An NAD‐specific glutamate dehydrogenase from cyanobacteria Identification and properties
Author(s) -
Chávez S.,
Candau P.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80719-j
Subject(s) - nad+ kinase , cyanobacteria , glutamate dehydrogenase , biochemistry , chemistry , glutamate receptor , dehydrogenase , identification (biology) , enzyme , biology , bacteria , botany , genetics , receptor
The unicellular cyanobacterium Synechocystis sp. PCC 6803 presents a hexameric NAD‐specific glutamate dehydrogenase with a molecular mass of 295 kDa. The enzyme differs from the NADP‐glutamate dehydrogenase found in the same strain and is coded by a different gene. NAD‐glutamate dehydrogenase shows a high coenzyme specificity, catalyzes preferentially glutamate formation and presents K m values for ammonium, NADH and 2‐oxoglutarate of 4.5 mM, 50 μM and 1.8 mM respectively. An animating role for the enzyme is discussed.