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Nucleosomes occurring in protein‐free hybridoma cell culture Evidence for programmed cell death
Author(s) -
Franěk František,
Dolníková Jana
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80705-8
Subject(s) - microbiology and biotechnology , dna fragmentation , nucleoprotein , fragmentation (computing) , programmed cell death , biology , apoptosis , moiety , cell culture , monoclonal antibody , dna , chromatin , antibody , chemistry , biochemistry , genetics , stereochemistry , ecology
In addition to monoclonal immunoglobulin. two kinds of nucleoproteins. NP1 and NP2, were isolated from the supernatants of hybridoma cultures set up in a protein‐free medium. As shown by SDS‐electrophoresis the two nucleoproteins shared a set or proteins (apparent M r 11000 to 15000). and differed in the DNA moiety (∼ 150 bp in NP1. ∼ 350 bp in NP2). The amino acid composition of the protein moiety confirmed the nucleosomal origin of NP1 and NP2. The findings support the view that in hybridoma cultures the cells undergo death by apoptosis, i.e. a programmed process characterized by initial fragmentation of chromatin.