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Antigen receptor‐mediated calcium signals in B cells as revealed by confocal fluorescence microscopy
Author(s) -
Yamada Hiroshi,
Mizuguchi Junichiro,
Nakanishi Mamoru
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80696-z
Subject(s) - confocal microscopy , confocal , fluorescence microscope , fluorescence , chemistry , biophysics , calcium , microbiology and biotechnology , receptor , antigen , biology , biochemistry , immunology , optics , physics , organic chemistry
A confocal fluorescence microscope was used to study the antigen receptor‐mediated calcium signals in B cells. Anti‐IgD binding to B lymphoma cells (BAL17) increased the intracellular calcium concentration with short lag times. Confocal fluorescence images of the fluo‐3‐loaded BAL17 cells showed that the intracellular calcium ion concentrations increased non‐homogeneously, suggesting that the calcium signals transferred not only to the cytoplasm but also to the nucleus.