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A novel γ subunit of the GABA A receptor identified using the polymerase chain reaction
Author(s) -
Wilson-Shaw Donna,
Robinson Misi,
Gambarana Carla,
Siegel Ruth E.,
Sikela James M.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80687-x
Subject(s) - protein subunit , specificity factor , gamma subunit , interleukin 10 receptor, alpha subunit , gamma aminobutyric acid receptor subunit alpha 1 , gabaa receptor , interleukin 5 receptor alpha subunit , complementary dna , microbiology and biotechnology , cys loop receptors , biology , peptide sequence , beta (programming language) , interleukin 12 receptor, beta 1 subunit , g alpha subunit , alpha (finance) , receptor , polymerase , biochemistry , gene , medicine , construct validity , nursing , nicotinic agonist , rna dependent rna polymerase , nicotinic acetylcholine receptor , computer science , patient satisfaction , programming language
We have utilized a polymerase chain reaction (PCR) strategy to identify a novel subunit, γ3, of the GABA A receptor. The γ3 cDNA encodes a mature protein of 450 amino acids that contains structural features typically conserved among subunits of the GABA A receptor family. The γ3 subunit shares approximately 66% sequence identity with the γ2 subunit but only 38% and 29% with α1 and β1 subunits, respectively. Localization of the γ3 mRNA indicates that it is widely distributed throughout the mouse brain in a pattern similar to that observed for mRNAs encoding the γ2 subunits.