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Sub‐picosecond dynamics of excited state of primary electron donor in reaction centers of Rhodopseudomonas viridis as revealed by hole burning at 1.7K broad and narrow holes
Author(s) -
Ganago A.O,
Shkuropatov A.Ya,
Shuvalov V.A
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80684-u
Subject(s) - excited state , picosecond , absorbance , phonon , full width at half maximum , photosynthetic reaction centre , chemistry , electron , analytical chemistry (journal) , atomic physics , molecular physics , materials science , electron transfer , photochemistry , physics , optics , condensed matter physics , laser , quantum mechanics , chromatography
Within the Q γ band of the primary electron donor (P), the spectra of absorbance changes due to the formation of a state P + Q A − (Q A is the primary quinone) at 1.7K in Rhodopseudomous viridis reaction centers excited at 1014 nm have been shown to involve two spectral features characterized by: (i) a progression of broad (170–190 cm −1 ) Gaussian vibronic bands ( S ‐factor = 1.4) separated by 150 cm −1 and (ii) a ‘narrow’ structure near 1014 nm, characterized by 0‐0 transition at 1014 nm with a width of ∼50 cm −1 and 0–1 transition at 1000 nm with the width of ∼100 cm −1 , and S ‐factor = 0.9. The width of 50 cm −1 can be related to either zero‐phonon hole (ZPH) width or the structure involving phonon wings and ZPH being unresolved. Since dichroic value (∼0.37) is unvarying over the P band, the vibrations involved are totally symmetric. The ZPH (width of ∼3 cm −1 ) and phonon wings (frequency of ∼30 cm −1 ) are resolved within the P band near 1014 nm when the spectrum of Δ A due to the formation of bacteriopheophytin L − is measured at 1.7K.