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Molecular cloning of a novel putative G‐protein coupled receptor expressed during rat spermiogenesis
Author(s) -
Meyerhof Wolfgang,
Müller-Brechlin Renate,
Richter Dietmar
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80674-r
Subject(s) - complementary dna , biology , northern blot , microbiology and biotechnology , in situ hybridization , spermiogenesis , cdna library , sertoli cell , messenger rna , receptor , spermatogenesis , expression cloning , gene , genetics , endocrinology
A cDNA clone encoding a novel putative G‐protein coupled receptor has been isolated from a rat testis cDNA library using a PCR‐amplified cDNA fragment as a hybridization probe. Northern blot analysis reveals that a corresponding 1.5 kb mRNA is exclusively expressed in testis. By in situ hybridization experiments this mRNA has been localized in spermatocytes and spermatids but not in spermatogonia, Leydig or sertoli cells. Ontogenic studies show that expression of the receptor‐encoding mRNA and sexual maturation are correlated reaching highest levels during the second and third months. Although the ligand for this receptor has not yet been identified, this receptor may play a role during reproduction.