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Energetic cooperation via ion‐permeable junctions in mixed animal cell cultures
Author(s) -
Aslanidi K.B.,
Boitsova L.Ju.,
Chailakhyan L.M.,
Kublik L.N.,
Marachova I.I.,
Potapova T.V.,
Vinogradova T.A.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80612-7
Subject(s) - ouabain , intracellular , fibroblast , cell culture , biophysics , cell , gap junction , chemistry , baby hamster kidney cell , ion , microbiology and biotechnology , biology , biochemistry , sodium , genetics , organic chemistry
Low ouabain concentration (1 × 10 −4 M) is shown to decrease intracellular K + (K + in ) and to increase intracellular Na + (Na + in ) in human fibroblast cell cultures. The same ouabain concentration was without effect upon K + in ad Na + in in rodent cultures such as BHK‐21, mouse fibroblasts and rat glyoma C6 cells. K + in and Na + in in the mixed cultures of human and BHK‐21 fibroblasts or human and mouse fibroblasts were found to be resistant to 1 × 10 −4 M ouabain whereas that of the mixtures of human and rat glyoma C6 cells proved to be ouabain‐sensitive. The gap‐junction‐mediated dye transfer was revealed between human and BHK‐21 cells. Such an effect was very small in the human‐C6 cell mixed culture. It is concluded that cells with active ion pumps can support the maintenance of K + and Na + gradients in cells with inactive pumps, provided that effective ion transport via gap junctions takes place.