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Differential synthesis by cultured atrial and ventricular rat cardiac myocytes of myosin light chain isoforms
Author(s) -
Brand Thomas,
Milting Hendrik,
Zippel Monika,
Jockusch Harald
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80610-f
Subject(s) - myh6 , gene isoform , myocyte , myosin , medicine , myosin light chain kinase , methionine , chemistry , endocrinology , microbiology and biotechnology , biology , myh7 , biochemistry , amino acid , gene
Dissociated cells from neonatal rat atria and ventricles were cultured in monolayers for 3 days. Newly synthesized 35 S‐methionine labeled myosin light chain isoforms ALC‐1, ALC‐2 (atrial) and VLC‐1, VLC‐2 (ventricular) were identified on 2D gels, and their pattern of synthesis was compared to that of myocard fragments immediately after explantation. ALCs were synthesized in 5‐ to 10‐fold excess over VLCs by atrial cultures, whereas the converse was true for ventricular cultures, with two exceptions: one third of the LC‐1 synthesized by ventricular fragments was ALC‐1, and dissociated atrial cells synthesized very little LC‐2 of either isoform. The former finding corresponds to the relatively high proportion of ALC‐1 in neonatal ventricular tissue. We conclude that the regional programme of LC isoform expression is basically retained after tissue explantation and even after dissociation and culturing of cardiac myocytes.