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Diazipine, a novel photoaffinity probe for dihydropyridine receptors of calcium channels
Author(s) -
Taki Motohiko,
Nakayama Hitoshi,
Kanaoka Yuichi
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80602-y
Subject(s) - dihydropyridine , dithiothreitol , chemistry , photoaffinity labeling , biophysics , binding site , receptor , calcium , ion channel , dhps , calcium channel , biochemistry , biology , enzyme , organic chemistry , malaria , immunology , plasmodium falciparum
A new 1,4‐dihydropyridine photoaffinity ligand, [ 3 H]diazipine, has been assessed by binding and photolabeling, and compared with a currently used [ 3 H]Diazipine reversibly binds to skeletal muscle Ca 2+ channels with a similar affinity of [ 3 H]azidopine, but [ 3 H]diazipine labels the channels two times more efficiently and no release of the incorporated amount is observed after dithiothreitol treatment.