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After microinjection hemimethylated DNA is converted into symmetrically methylated DNA before DNA replication
Author(s) -
Sandberg G.,
Guhl E.,
Graessmann M.,
Graessmann A.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80599-x
Subject(s) - dna , dna replication , dna methylation , eukaryotic dna replication , microbiology and biotechnology , biology , dna clamp , dna polymerase ii , genetics , chemistry , gene , gene expression , polymerase chain reaction , reverse transcriptase
In this investigation we analysed the maintenance methylation activity of the mammalian cell DNA methyltransferase by microinjection of hemimethylated HSV‐tk DNA into thymidine kinase‐negative rat 2 cells. We found that the hemimethylated DNA was efficiently converted into symmetrical methylated molecules before DNA replication. Furthermore, integration of the trans ‐DNA into the host genome is an early event after gene transfer.