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Localization of metallothionein in nuclei of growing primary cultured adult rat hepatocytes
Author(s) -
Tsujikawa Kazutake,
Imai Takumi,
Kakutani Makoto,
Kayamori Yuzo,
Mimura Tsutomu,
Otaki Noriko,
Kimura Masami,
Fukuyama Ryuichi,
Shimizu Nobuyoshi
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80597-v
Subject(s) - metallothionein , cytoplasm , subcellular localization , staining , biology , microbiology and biotechnology , antiserum , cell nucleus , hepatocyte , immunofluorescence , chemistry , biochemistry , antibody , in vitro , immunology , genetics , gene
In primary cultured adult rat hepatocytes stimulated by epidermal growth factor and insulin, dramatic changes in the subcellular distribution of metallothionein were clarified by indirect immunofluorescence using antisera specific for this protein. Metallothionein was detected only in the cytoplasm of cultured hepatocytes in the G 0 and G 1 phases, but was concentrated in the cell nuclei in the early S phase. The strongest staining pattern in the nuclei was observed 12 h after stimulation. Subsequently, the intensity of metallothionein staining in the nuclei decreased. These results suggests that primary cultured hepatocytes are suitable for examining the relation between subcellular localization of metallothionein and cell growth.