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Dystrophin constitutes 5% of membrane cytoskeleton in skeletal muscle
Author(s) -
Ohlendieck Kay,
Cambell Kevin P.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80595-t
Subject(s) - dystrophin , sarcolemma , skeletal muscle , cytoskeleton , duchenne muscular dystrophy , itga7 , utrophin , microbiology and biotechnology , myocyte , muscular dystrophy , biology , chemistry , anatomy , cell , biochemistry , genetics
Dystrophin, which is absent in skeletal muscle of Duchenne muscular dystrophy patients, has not been considered to play a major structural role in the cell membrane of skeletal muscle because of its low abundance (∼ 0.002% of total muscle protein). Here, were have determined the relative abundance of dystrophin in a membrane cytoskeleton preparation and found that dystrophin constitutes approximately 5% of the total membrane cytoskeleton fraction of skeletal muscle sarcolemma. In addition, dystrophin can be removed from surcolemma by alkaline treatment. Thus, our results have demonstrated that dystrophin is a major component of the subsurcolemmal cytoskeleton in skeletal muscle and suggest that dystrophin could play a major structural role in the cell membrane of skeletal muscle.