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rig encodes ribosomal protein S15 The primary structure of mammalian ribosomal protein S15
Author(s) -
Kitagawa Motoo,
Takasawa Shin,
Kikuchi Norihisa,
Itoh Takako,
Teraoka Hiroshi,
Yamamoto Hiroshi,
Okamoto Hiroshi
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80590-y
Subject(s) - ribosomal protein , 30s , biology , messenger rna , complementary dna , ribosomal rna , protein primary structure , microbiology and biotechnology , peptide sequence , nucleic acid sequence , biochemistry , ribosome , gene , rna
rig , a gene originally isolated from a rat insulinoma cDNA library, codes for a basic 145 amino acid protein [(1986) Diabetes 35, 1178–1180]. Here we show that the immunoreactivity to a monocional antibody against the deduced rig protein and the translation product of rig mRNA comigrated with ribosomal protein S15. The amino acid sequence of ribosomal protein S15 purified from rat liver coincided with that deduced from the nucleotide sequence of rig mRNA, but there were indications that the initiator methionine was removed and the succeeding alanyl residue was monoacetylated. From these results, we conclude that the product of rig is ribosomal protein S15.