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Isolation of a novel arachidonic acid metabolite 3‐hydroxy‐5,8,11,14‐eicosatetraenoic acid (3‐HETE) from the yeast Dipodascopsis uninucleata UOFs‐Y128
Author(s) -
van Dyk M.S.,
Kock J.L.F.,
Coetzee D.J.,
Augustyn O.P.H.,
Nigam S.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80586-r
Subject(s) - metabolite , arachidonic acid , chemistry , yeast , chromatography , high performance liquid chromatography , mass spectrometry , thin layer chromatography , biochemistry , enzyme
When arachidonic acid (AA) is added to the yeast Dipodascopsis uninucleata UOFS Y128, one of the major metabolites isolated and purified with the help of thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) is 3‐hydroxy‐5,8,11,14‐eicosatetraenoic acid (3‐HETE). The structure of this new AA metabolite was elucidated mainly by electron impact (EI) mass spectrometry (MS). Strikingly, the formation of this new metabolite was found to be inhibited by aspirin.