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Increased responsiveness of rat mast cells to compound 48/80 due to removal of extracellular magnesium Effects of ouabain and EGTA
Author(s) -
Bertelsen Henrik,
Johansen Torben
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80584-p
Subject(s) - egta , ouabain , magnesium , calcium , divalent , chemistry , secretion , extracellular , endocrinology , medicine , biophysics , biochemistry , biology , sodium , organic chemistry
A decreased secretory response of mast cells to compound 40/80 (12% of control value) after preincubation of the cells with magnesium but without calcium was partially restored by removal of magnesium. EGTA (10 μM) blocked the restoration and decreased the restored secretory activity again, while this was further increased by ouabain (1 mM). Furthermore, ouabain completely restored the decreased secretion (50% of control value) due to preincubation without the divalent cations. This may indicate that magnesium influences a pool of cellular calcium that is involved in the stimulus‐secretion coupling and is available to EGTA, and ouabain did not counteract the inhibitory mechanism or magnesium.