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Cloning of the protein D2 gene of Pseudomonas aeruginosa and its functional expression in the imipenem‐resistant host
Author(s) -
Yoneyama Hiroshi,
Nakac Taiji
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80582-n
Subject(s) - imipenem , bacterial outer membrane , pseudomonas aeruginosa , mutant , microbiology and biotechnology , cloning (programming) , gene , plasmid , biology , integral membrane protein , membrane protein , chemistry , bacteria , escherichia coli , biochemistry , genetics , membrane , computer science , programming language
Protein D2 forms the water‐filled pore across the outer membrane of Pseudomonas aeruginosa and allows the penetration of imipenem. We cloned the protein D2 gene by the antibody screening technique. When the imipenem‐resistant mutant lacking protein D2 harbored the plasmid with the cloned D2 gene, the mutant overproduced protein D2 in the outer membrane. These transformants exhibited fully‐restored imipenem susceptibility. The results prove unequivocally that protein D2 forms the imipenem‐permeable pore in the P. aeruginosa outer membrane.