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cDNA cloning and chromosomal localization of the human α‐adrenergic receptor kinase
Author(s) -
Benovic J.L.,
Stone W.C.,
Huebner K.,
Croce C.,
Caron M.G.,
Lefkowitz R.J.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80568-n
Subject(s) - complementary dna , cdna library , microbiology and biotechnology , biology , beta adrenergic receptor kinase , locus (genetics) , gene , receptor , genetics , g protein coupled receptor
The β‐adrenergic receptor kinase (βARK) mediates agonist‐dependent phosphorylation of the β 2 ‐adrenergic and related G protein‐coupled receptors. A cDNA encoding bovine βARK has recently been isolated. In this work we have isolated a cDNA encoding human βARK from a retinal cDNA library. The cDNA encodes a protein of 689 amino acids with an overall 98.0% amino acid and 92.5% nucleotide identity with bovine βARK. Chromosomal location of the human βARK gene was determined by correlating the presence of the βARK locus with retention of a specific human chromosome in a rodent‐human hybrid panel. This analysis revealed that the human βARK locus segregated with the long arm of chromosome 11, centromeric to 11q13.