Premium
The three‐dimensional structure of proteasomes from Thermoplasma acidophilum as determined by electron microscopy using random conical tilting
Author(s) -
Hegerl Reiner,
Pfeifer Günter,
Pühler Gabriela,
Dahlmann Burkhardt,
Baumeister Wolfgang
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80567-m
Subject(s) - thermoplasma acidophilum , conical surface , electron micrographs , electron microscope , crystallography , cylinder , chemistry , materials science , nanotechnology , biophysics , biology , physics , optics , biochemistry , mathematics , geometry , composite material , enzyme
The three‐dimensional structure of proteasomes from the archaebacterium Thermoplasma acidophilum has been determined to a resolution of approximately 2 nm from electron micrographs of negatively stained preparations using the method of random conical tilting. The particles turn out to be essentially cylinder‐shaped barrels, 15 nm long and 11 nm wide, enclosing a tripartite inner compartiment. An account is given of some of the present limitations which prevent to attain a higher resolution and possible ways to overcome these limitations are indicated.