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Cultured human keratinocytes cannot metabolize vitamin D 3 to 25‐hydroxyvitamin D 3
Author(s) -
MacLaughlin Julia A.,
Castonguay Maurice A.,
Holick Michael F.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80525-8
Subject(s) - high performance liquid chromatography , vitamin d and neurology , incubation , chemistry , chromatography , metabolism , vitamin , biochemistry , biology , endocrinology
The metabolism of [ 3 H] vitamin D 3 was studied in cultured human keratinocytes (CHK). Intact CHK were incubated for 1, 6, 12, 24 and 48 h with [ 3 H] vitamin D 3 and the lipid soluble fractions from the media and cells were extracted by high‐performance liquid chromatography (HPLC). Vitamin D 3 and its metabolites, 25‐OH‐D 3 , 24,25(OH) 2 D 3 were added to the extracts, as markers, prior to HPLC, HPLC analysis of the lipid extracts did not reveal any monohydroxylated metabolites. CHK incubated for one hour with [ 3 H]25‐OH‐D 3 showed a 10±4% conversion to [ 3 H]1,25,(OH) 2 D 3 whereas no conversion to [ 3 H]1,25(OH) 2 D 3 was observed in control CHKs that were boiled prior to incubation with [ 3 H]25‐OH‐D 3 . These findings suggest that cultured neonatal keratinocytes are incapable of metabolizing vitamin D 3 to 25‐OH‐D 3 .