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Recombinant aequorin as a probe for cytosolic free Ca 2+ in Escherichia coli
Author(s) -
Knight Marc R.,
Campbell Anthony K.,
Smith Steven M.,
Trewavas Anthony J.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80524-7
Subject(s) - aequorin , escherichia coli , recombinant dna , cytosol , calcium , phagocytosis , chemistry , biochemistry , bioluminescence , fluorescence , biophysics , microbiology and biotechnology , biology , enzyme , physics , organic chemistry , quantum mechanics , gene
We describe a novel and simple method for the measurement of bacterial cytosolic free calcium ([Ca 2+ ] i ) using recombinant acquorin reconstituted within live bacterial cells. Using this method we have measured the effects of external calcium, complement, phagocytosis and antibiotics on the [Ca 2+ ] i of Escherichia coli . In principle this method should be applicable to any genetically transformable organism and should suffer fewer problems than fluorescent dyes for subcellular calcium measurement.