Premium
Both D factor/LIF and IL‐6 inhibit the differentation of mouse teratocarcinoma F9 cells
Author(s) -
Hirayoshi Kazunori,
Tsuru Akio,
Yamashita Michiaki,
Tomida Mikio,
Yamamoto-Yamaguchi Yuri,
Yasukawa Kiyoshi,
Hozumi Motoo,
Goeddel D.V.,
Nagata Kazuhiro
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80523-6
Subject(s) - leukemia inhibitory factor , teratocarcinoma , microbiology and biotechnology , retinoic acid , biology , cell culture , phorbol , chemistry , cellular differentiation , cancer research , interleukin 6 , cytokine , immunology , signal transduction , biochemistry , protein kinase c , gene , genetics
Differentation‐stimulating factor (D factor)/leukemia inhibitory factor (LIF) and IL‐6 are reported to be cytokines having multifaced functions including the induction of differentation in mouse myeloid leukemia MI cells. We here report that both D factor/LIF and IL‐6 inhibit the differentation of mouse teratocarcinoma F9 cells induced by retinoic acid alone or combined with dibutyryl cAMP. From the microscopic observation as well as Northern blot analysis using cDNA probes encoding several marker proteins for differentiation of F9 cells, we conclude that D factor/ LIF and IL‐6 are functionaly closely related in the induction of differentation in MI cells in the inhibition of F9 differentiation.