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Cloning of human α1(X) collagen DNA and localization of the COL10A1 gene to the q21–q22 region of human chromosome 6
Author(s) -
Apte Suneel,
Mattei Marie-Genevieve,
Olsen Bjorn R.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80521-4
Subject(s) - microbiology and biotechnology , biology , gene , locus (genetics) , southern blot , genetics , genomic dna , human genome , chromosome 21 , nucleic acid sequence , molecular cloning , chromosome , genome , peptide sequence
With consensus primers based upon the nucleotide sequence of the chicken α1(X) collagen gene, we have used PCR with human genomic DNA as template to isolate a 289 bp fragment coding for part of the carboxyl non‐triple helical domain of the human α1(X) gene. We have demonstrated the presence of the sequence of the PCR clone within the human genome by partial sequence analysis of a 1 kb Hin dIII genomic DNA fragment that hybridized with the PCR clone. Furthermore, using the PCR clone as a probe for in situ hybridization of human metaphase chromosome spreads, and for Southern analysis of a panel of human—hamster somatic cell hybrid DNAs, we have assigned he locus for the α1(X) gene to the q‐21–q22 region of human chromosome 6.