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Effects of neutrophil adherence on the characteristics of receptors for tumor necrosis factor‐α
Author(s) -
Chamba Anita,
Stockley Robert A.,
Burnett David
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80516-6
Subject(s) - tumor necrosis factor alpha , receptor , fibronectin , chemistry , cytokine , adhesion , cell surface receptor , microbiology and biotechnology , recombinant dna , immunology , cell , biology , biochemistry , organic chemistry , gene
Human recombinant [ 125 ]TNF‐α was incubated with non‐adherent human neutrophils, cells adherent to fibronectin‐coated plastic, or adherent cells scraped into suspension (post‐adherent). Binding of TNF to all cells increased with doses of added TNF but adherent cells little TNF, Binding of TNF by post‐adherent cells was greater than when adherent, but still significantly less than of non‐adhered neutrophils, suggesting that TNF receptors were relocated on the adherent surface of neutrophils. Scatchard analysis showed that adherent cells expressed significantly fewer TNF receptors, but of higher affinity, then non‐adherent cells. The results suggest that altered expression of TNF receptors might contribute to the differential effects of TNF on adherent and non‐adherent neutrophils.