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Differential regulation of MyoD and myogenin mRNA levels by nerve induced muscle activity
Author(s) -
Witzemann Veit,
Sakmann Bert
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80490-t
Subject(s) - myogenin , myod , denervation , neuromuscular junction , acetylcholine receptor , reinnervation , neuromuscular transmission , biology , microbiology and biotechnology , endocrinology , synapse , medicine , myogenesis , neuroscience , chemistry , skeletal muscle , receptor , biochemistry
The levels of mRNAs coding for the myogenic factors MyoD and myogenin were measured during synapse formulation in developing muscle and in adult muscle, after denervation and reinnervation and after muscle paralysis induced by blocking of neuromuscular transmission by neurotoxins known to alter the density and localization of synaptic proteins such as the acetylcholine receptor (AChR). The mRNA levels of both factors depend on usage of the neuromuscular synapses, but they change to different extents. Myogenin mRNA levels decrease drastically with innervation and increase strongly following blocking of transmission whereas the level of MyoD mRNA showed only a small decrease in response to innervation, denervation of muscle paralysis by neurotoxins. Neither mRNA showed a synapse‐related cellular distribution. The results suggest that nerve‐induced electrical muscle activity determines the cellular ratio of MyoD and myogenin mRNAs in adult muscle.