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Calpactin‐depleted cytosolic proteins restore Ca 2+ ‐dependent secretion to digitonin‐permeabilized bovine chromaffin cells
Author(s) -
Wu You Neng,
Wagner Paul D.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80476-j
Subject(s) - digitonin , cytosol , secretion , secretory protein , exocytosis , microbiology and biotechnology , chemistry , biochemistry , biology , enzyme
Incubation of digitonin‐permeabilized bovine chromaffin cells results in a loss of Ca 3+ ‐dependent catecholamine secretion. The addition of cytosolic proteins prevents this loss of secretory activity. It has been proposed that calpactin might be the protein which is responsible for preventing this loss of activity. The experiments described in this paper show that cytosolic proteins which have been depleted of calpactin are as effective as control cytosolic proteins in preventing the loss of Ca 2+ ‐dependent secretion. Thus, a cytosolic protein(s) other than calpactin appears to be responsible for preventing this loss of secretory activity.