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Identification of Nuclear Factor IV/Ku autoantigen in a human 2D‐gel protein database Modification of the large subunit depends on cellular proliferation
Author(s) -
Stuiver Maarten H.,
Celis Julio E.,
van der Vliet Peter C.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80474-h
Subject(s) - protein subunit , aphidicolin , microbiology and biotechnology , dna , hela , dna replication , nuclear protein , biology , cell growth , dna synthesis , thymidine , cell , chemistry , biochemistry , gene , transcription factor
Nuclear Factor IV (NFIV)is a heterodimeric DNA‐binding protein from HeLa cells, recognizing molecular ends and is identical to the autoantigenic target Ku. We have identified the two NFIV/Ku subunits, by comigration, in the 2D‐gel database of transformed human amnion cell (AMA) proteins. We observed that the large subunit or NFIV/Ku consists of at least 3 charge variants that correspond to SSP IEFs 5705 (81.2 kDa, p I 5.74), 6707 (81.2 kDa, p I 5.67) and 6706 (81.9 kDa, p I 5.60) in the AMA catalogue. The relative amounts of the 2 major variants (IEFs 5705 and 6707) was dependent on the state of cell proliferation. Inhibition of DNA‐synthesis by hydroxyurea also changed the relative levels of the variants, whereas aphidicolin or a thymidine block had no effect. These results suggest a possible role for NFIV/Ku in DNA replication.