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A rapid purification method for neurogranin, a brain specific calmodulin‐binding protein kinase C substrate
Author(s) -
Deloulme Jean Christophe,
Sensenbrenner Monique,
Baudier Jacques
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80473-g
Subject(s) - neurogranin , calmodulin , protein kinase c , protein kinase a , biochemistry , chemistry , substrate (aquarium) , kinase , biology , enzyme , ecology
A rapid purifiacation method is reported for bovine brain neurogranin, a calmodulin‐binding protein kinase C (PKC) substrate. This method takes advantage of the fact that the protein remains soluble in 2.5% perchlorie acid (PCA) and that it binds to a calmodulin‐Sepharose column in the absence of calcium. Other PKC substrate proteins that remain to be to be identified were also found to share these two properties, suggesting that a class of calmodulin‐binding PKC substrates may exist in the brain.