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Production of a full length Tat protein in E. coli and its purification
Author(s) -
Armengaud Jean,
de Nuova Perez Lidia,
Lemay Piere,
Masson Jean-Michel
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80467-h
Subject(s) - chemistry , escherichia coli , biochemistry , gene
A full length tat gene was constructed by a combination of a polymerase chain reaction (PCR) for the first exon and chemical synthesis for the second exon. This gene was expressed in E. Coli under the control of the strongly regulated ara B promoter, either directly or fused to a secretion signal encoding sequence. We the defined a rapid, three‐step procedure for the purification of the Tat protein