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Molecular cloning of cDNA and a chromosomal gene encoding GPE1‐BP, a nuclear protein which binds to granulocyte colony‐stimulating factor promoter element 1
Author(s) -
Nishizawa Mikio,
Wakabayashi-Ito Noriko,
Nagata Shigekazu
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80452-9
Subject(s) - microbiology and biotechnology , complementary dna , leucine zipper , gene , biology , exon , nuclear protein , transcription factor , genetics
Granulocyte colony‐stimulating factor (G‐CSF) is produced from macrophages in response to lipopolysaccharide (LPS). GPE1, a cis ‐acting element of the G‐CSF gene promoter, functioned as an LPS‐inducible element. We isolated cDNA and a chromosomal gene encoding the mouse GPEI‐binding protein (GPE1‐BP). A 150‐amino acid protein deduced from the cDNA has a basic domain and a leucine zipper motif, and seems to be identical to that of recently isolated Ig/EBP. The nuclear extract from COS cells transfected with the cDNA showed GPE1‐binding activity. Transcripts were ubiquitously detected, and may be spliced from two exons of a single gene.