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Extracellular ATP itself elicits superoxide generation in guinea pig peritoneal macrophages
Author(s) -
Nakanishi Masayuki,
Takihara Hironori,
Minoru Yoshida,
Yagawa Katsuro
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80451-8
Subject(s) - extracellular , superoxide , pertussis toxin , guinea pig , calcium , cytosol , chemistry , biochemistry , phorbol , microbiology and biotechnology , endocrinology , biology , enzyme , protein kinase c , g protein , signal transduction , organic chemistry
Extracellular ATP itself elicited the generation of superoxide (O − 2 ) in guinea pig peritoneal macrophages associated with an increase in cytosolic calcium ([Ca 2+ ]). The ATP‐Induced O − 2 generation was completely inhibited by pretreatment with pertussis toxin (PT) accompanied by the suppression of (Ca 2+ ), mobilization. Pre‐exposure to a small amount of phorbol myristate acetate (PMA) primed the ATP‐induced generation of O − 2 without it change of (Ca 2+ ). The results suggest that ATP‐induced O − 2 generation is mediated by (Ca 2+ ), mobilization and by PT‐sensitive G protein.