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Role of a GC‐rich motif in transcription regulation of the adenovirus type 2 IVa2 promoter which lacks typical TATA‐box element
Author(s) -
Tamura Taka-aki,
Mikoshiba Katsuhiko
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80450-h
Subject(s) - tata box , promoter , caat box , response element , microbiology and biotechnology , transcription (linguistics) , transcription factor , biology , e box , gene , genetics , gene expression , linguistics , philosophy
Promoter of the adenovirus type 2 IVa2 (IVa2) gene does not have a TATA‐box element, hence it is considered to be a model of cellular TATA‐less promoters. In vitro transcription of the IVa2 promoter in HeLa cell extracts identified a proximal cis ‐element around −40 relative to the transcription start site. DNase I footprint analysis revealed the presence of a DNA‐binding factor which interacts with sequences ACCCCTCCCACTTAG at −50. Competition footprint analysis and sequence comparisons showed that the IVa2 proximal promoter element is equivalent to the GC‐box, the Spl‐binding site.