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A 53 kDa protein binds to the negative regulatory region of JC virus early promoter
Author(s) -
Sharma Arun K.,
Kumar Gyanendra
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80409-v
Subject(s) - hela , microbiology and biotechnology , biology , promoter , virus , gene , cell , gene expression , virology , biochemistry
Using gel retardation and photocrosslinking experiments, we have identified proteins of about 53 kDa in size in both rat glioma (C6) and cervical carcinoma (HeLa) cell extracts which bind to the negative regulatory region or JC virus (JCV) early promoter. The glial cell protein binds to its cognate promoter element with lesser affinity when compared to the protein present in HeLa cells. Futher, these proteins interacted differentially to an oligonucleotide, containing the neighboring cis ‐acting domain, which is recognized by nuclear factor 1 (NFI). These findings suggest that the interactions of the 53 kDa HeLa protein may contribute to the regulation of JCV early promoter in HeLa cells.