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Modification of the stability of phospholipase A2 by charge engineering
Author(s) -
Pickersgill Richard W.,
Sumner Ian G.,
Collins Margaret E.,
Warwicker Jim,
Perry Barry,
Bhat K.Mahalingeshwara,
Goodenough Peter W.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80397-l
Subject(s) - mutant , stability (learning theory) , protein stability , charge (physics) , chemistry , protein engineering , biophysics , globular protein , chemical physics , crystallography , biology , physics , biochemistry , computer science , enzyme , gene , quantum mechanics , machine learning
Electrostatic interactions play an important role in stabilizing the folded conformation of globular proteins. Here we predict the change in stability of charge engineered mutants, construct these mutants and compare the predicted change in stability with that observed. The change in stability was correctly predicted for two of the three mutants and the factors responsible for the discrepancy between observation and prediction for the third mutant are discussed.