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On the functional form of transcortin‐recognizing subunit of transcortin membrane receptor
Author(s) -
Krupenko Sergey A.,
Avvakumov George V.,
Strel'chyonok Oleg A.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80381-c
Subject(s) - transcortin , sialoglycoprotein , membrane , protein subunit , receptor , biophysics , biochemistry , tetramer , cell surface receptor , transmembrane protein , chemistry , biology , globulin , endocrinology , gene , enzyme
Complex formation between transcortin and the 20 kDa sialoglycoprotein from the plasma membrane of human decidual endometrium (presumably a transcortin‐recognizing subunit of transcortin membranes receptor) was studied using cross‐linking reagents. The action of 1,5‐difluoro‐3,4‐dinitrobenzene (DFDNB) on a solution of 125 I‐labelled 20 kDa sialoglycoprotein and unlabelled transcortin resulted in the formation of two 125 I‐containing species that corresponded to covalently linked complexes of one transcortin molecule and either 2 or 4 molecules of the labelled membrane sialoglycoprotein. Only the latter complex was observed when the endometrium membranes were incubated with [ 25 I]transcortin and treated with DFDNB. This suggests that the functional form of transcortin‐recognizing subunit of the membrane receptor is a tetramer.