Premium
Interaction of mitochondrial creatine kinase with model membranes A monolayer study
Author(s) -
Rojo Manuel,
Hovius Ruud,
Demel Rudy,
Wallimann Theo,
Eppenberger Hans M.,
Nicolay Klaas
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80374-c
Subject(s) - monolayer , creatine kinase , membrane , phospholipid , inner mitochondrial membrane , mitochondrion , chemistry , biophysics , surface pressure , biochemistry , biology , physics , mechanics
The interaction of mitochondrial creatine kinase (Mi‐CK; EC 2.7.3.2) with phospholipid monolayers and spread mitochondrial membranes at the air/water interface has been investigated. It appeared that Mi‐CK penetrated into these monolayers as evidenced by an increase in surface pressure upon incorporation of Mi‐CK. The increase in surface pressure was dependent on (1) the amount and (2) the oligometric form of Mi‐CK in the subphase, as well as on (3) the initial surface pressure and (4) the phospholipid composition of the monolayer. In this experimental system Mi‐CK was able to interact equally well with both inner and outer mitochondrial membranes.