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What is the true ATPase activity of contracting myofibrils?
Author(s) -
Houadjeto Maurice,
Barman Tom,
Travers Franck
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80369-e
Subject(s) - myofibril , chemistry , atpase , biophysics , biochemistry , enzyme , biology
The ATPase activity of contracting myofibrils was obtained in a solvent of ionic contents that mimic in vivo conditions: pH 7.4 and 0.1 M potassium acetate. Contracting myofibrils are fleeting structures and their ATPase activity is of short duration: even at 4°C it was over after a reaction time of 1 s and rapid reaction equipment was needed for its study. The ATPase (1.1 s −1 ) was very similar to that of crosslinked actoS1 (1 s −1 ). It was 100 × that of relaxed myofibrils.