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The chloroplast gene for ribosomal protein CL23 is functional in tobacco
Author(s) -
Yokoi Fumiaki,
Tanaka Minoru,
Wakasugi Tatsuya,
Sugiura Masahiro
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80359-b
Subject(s) - pseudogene , gene , genetics , open reading frame , biology , ribosomal protein , chloroplast , ribosomal rna , ribosome , coding region , locus (genetics) , stop codon , start codon , nicotiana tabacum , transfer rna , chloroplast dna , peptide sequence , messenger rna , genome , rna
Chloroplast rp123 loci potentially coding for a polypeptide homologous to the E. Coli L23 ribosomal protein are frame‐shifted in spinach and several other plants, indicating that these loci are pseudogenes. In tobacco, tp123 constitutes a continuous open reading frame of 93 codons and its transcript initiates at least 66 bp upstream from the initiation codon. The N‐terminal amino acid sequence of a 13 kDa protein from the 50 S subunit of tobacco chloroplast ribosomes matches that derived from the tobacco rp123 locus. This shows that rp123 is a functional gene in tobacco.