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AMPA and kainate‐operated channels reconstituted in artificial bilayers
Author(s) -
Ambrosini A.,
Barnard E.A.,
Prestipino G.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80350-c
Subject(s) - kainate receptor , ampa receptor , ionotropic effect , excitatory postsynaptic potential , biophysics , glutamate receptor , chemistry , postsynaptic potential , neuroscience , nmda receptor , biochemistry , microbiology and biotechnology , receptor , biology
Catlonic channels which can be activated by α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionate (AMPA) and kainate play a key role in the generation of excitatory posisynaptic potentials in the brain of all vertebrates. On a protein carrying binding sites for both these ligands, affinity‐purified from the Xenopus nervous system, electrical measurements have been performed to investigate its functional properties after the pure complex had been incorporated into planar lipid bilayers. Domoate, AMPA or kainate added to the reconstituted protein activated cationic channels, which were blocked by typical antagonists for this neurotransmitter system. These data suggest that the reconstituted protein is an ionotropic receptor of the unitary non‐NMDA sybtype.