Premium
Di(1,N 6 ‐ethenoadenosine)5′, 5‴‐P 1 ,P 4 ‐tetraphosphate, a fluorescent enzymatically active derivative of Ap 4 A
Author(s) -
Rotilán P.,
Ramos A.,
Pintor J.,
Torres M.,
Miras-Portugal M.T.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80334-y
Subject(s) - fluorescence , chemistry , nucleotide , hydrolysis , stereochemistry , crystallography , physics , biochemistry , quantum mechanics , gene
Di(1,N 6 ‐ethenoadenosine) 5′, 5‴‐P 1 , P 4 ‐tetraphosphate, ϵ‐(Ap 4 A), a fluorescent analog of Ap 4 A has been synthesized by reaction of 2‐chloroacetaldehyde with Ap 4 A. At neutral pH this Ap 4 A analog presents characteristic maxima at 265 and 274 nm, shoulders at ca 260 and 310 nm and moderate fluorescence (λ exc 307 nm, λ em 410 nm). Enzymatic hydrolysis of the phosphate backbone produced a slight hyperchromic effect but a notorious increase of the fluorescence emission. Cytosolic extracts from adrenochromaffin tissue as well as cultured chromaffin cells were able to split ϵ(Ap 4 A) and catabolize the resulting ϵ‐nucleotide moieties up to ϵ‐Ado.