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Cloning and sequence analysis of complementary DNA encoding a precursor for chicken natriuretic peptide
Author(s) -
Akizuki Naoya,
Kangawa Kenji,
Minamino Naoto,
Matsou Hisayuki
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80331-v
Subject(s) - cloning (programming) , peptide , sequence (biology) , chemistry , dna , sequence analysis , molecular cloning , encoding (memory) , peptide sequence , microbiology and biotechnology , biology , biochemistry , gene , computer science , neuroscience , programming language
Chicken α‐natriuretic peptide (α‐chNP) has been identified in chicken heart, which showed higher homology to brain natriuretic peptide (BNP) than to atrial natriuretic peptide (ANP) [1]. Complementary DNA (cDNA) clone encoding a chNP precursor (pre‐chNP) precursor (pre‐chNP) was isolated from cardiac cDNA library and sequenced. Pre‐chNP was 140‐residue signal peptide at the N‐terminus and α‐chNP at the C‐terminus, and did not exhibit high homology to poreine BNP except for the C‐terminal region. However, a characteristic AT‐rich nucleotide sequence commonly found in mammalian BNPs was also present in the 3′‐untranslated region. Thus, chNP is concluded to be classified into the BNP‐type