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Direct cleavage of a DNA fragment by a bleomycin‐oligonucleotide derivative
Author(s) -
Sergeyev D.S.,
Godovikova T.S.,
Zarytova V.F.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80309-q
Subject(s) - oligonucleotide , cleavage (geology) , bleomycin , chemistry , dna , reagent , stereochemistry , microbiology and biotechnology , biochemistry , biology , organic chemistry , genetics , paleontology , chemotherapy , fracture (geology)
Bicomycin A 1 oligonucleotide derivative was used for direct cleavage of a DNA target. In the presence of Fe 2+ ions and 2‐mercaptoethanol, Blm‐R‐pd(CCAAACA) (I) damaged the target, pd(TGTTTGGCGAAGGA), with the yield of 80%, without affecting its own oligonucleotide tail. The sites of the cleavage were T ? ‐T ? and G ? ‐G ? . Unbound bleomycin A 5 , damaged the G 6 ‐G 7 ‐G 8 site. Reagent I formed more stable complementary complexes with the target than parent oligonucleotide (Δ T m =11°C)