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Identification of an additional gene belonging to the α 2 adrenergic receptor family in the human genome by PCR
Author(s) -
Chhajlani Vijay,
Rangel Ninfa,
Uhlén Staffan,
Wikberg Jarl E.S.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80301-i
Subject(s) - gene , biology , genetics , microbiology and biotechnology , interleukin 5 receptor alpha subunit , alpha (finance) , human genome , scn3a , interleukin 10 receptor, alpha subunit , genomic dna , alpha 1b adrenergic receptor , restriction map , genomic organization , cloning (programming) , genome , nucleic acid sequence , adrenergic receptor , g alpha subunit , receptor , protein subunit , beta 3 adrenergic receptor , medicine , construct validity , nursing , computer science , patient satisfaction , programming language
We here describe the cloning of an additional gene, called α 2 ‐1.8, which is similar to the previously cloned human α 2 ‐adrenergic receptor located on chromosome 4. The α 2 ‐1.8 gene was identified by using the polymerase chain reaction with primers specific for sequences in transmembrane regions 2 and 5 of the previously isolated human α 2 ‐C4 and α 2 ‐C10 adrenoceptor genes, which are localized on chromosomes 4 and 10, respectively. The new gene was identified by amplifying the 1.8 kb size fractionated region of Pst I restriction cut human genomic DNA. The previously cloned α 2 ‐C10 and α 2 ‐C4 genes were recovered at their expected locations, 0.96 and 5.9 kb, respectively. We have identified 387 bases of the new α 2 ‐1.8 gene, and its sequence is identical to the previously described α 2 ‐C4 gene, but is distinct from the α 2 ‐C10 and α 2 ‐C2 genes. Our results demonstrate that the α 2 ‐C4 adrenergic receptor exists in more than one copy in the human genome.