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Structure of adsorbed fibrinogen obtained by scanning force microscopy
Author(s) -
Wigren Roger,
Elwing Hans,
Erlandsson Ragnar,
Welin Stefan,
Lundström Ingemar
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80298-h
Subject(s) - fibrinogen , adsorption , microscopy , molecule , scanning force microscopy , chemistry , resolution (logic) , atomic force microscopy , crystallography , materials science , nanotechnology , optics , organic chemistry , biochemistry , computer science , physics , artificial intelligence
It is shown that scanning force microscopy (SFM), operated in the attractive mode, can be used to obtain high resolution pictures of adsorbed fibrinogen molecules on solid surfaces, without the need for staining or special microscope grids. SFM also reveals the three‐dimensional structure of the adsorbed molecules. Two forms of adsorbed fibrinogen are demonstrated on hydrophobic silicone dioxide surfaces; a trinodular about 60 nm long and a globular with about a 40 nm diameter. Polymeric networks formed after storage of the surface with adsorbed fibrinogen in PBS for 11 days are also shown. The SFM‐results for the trinodular structure suggest the existence of loops or peptide chains extending outside the basic structure of the fibrinogen molecule.