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Differential effect of extracellular calcium on the Na + ‐K + pump activity in intact polymorphonuclear leucocytes and erythrocytes
Author(s) -
Petersen Reno H.,
Knudsen Torben,
Johansen Torben
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80272-5
Subject(s) - calcium , extracellular , chemistry , sodium , calcium pump , biophysics , biochemistry , enzyme , biology , atpase , organic chemistry
The effect of extracellular calcium on the Na + ‐K + pump activity in human polymorphonuclear leucocytes and erythrocytes was studied and compared with the activity in mixed peritoneal leucocytes from rats. While there was maximal decrease in the pump activity (25–30%) of leucocytes from both rat and human by calcium 0.6 mM, a concentration of 0.1 mM caused a substantial decrease indicating a high sensitivity for extracellular calcium. In contrast, calcium had no effect on the pump activity in crythrocytes. The effect of calcium on the pump activity in leucocytes may be due to regulation of the influx of sodium across the plasma membrane, since in human leucocytes calcium had no effect on the pump activity if the cells were loaded with sodium.