Premium
cDNA cloning of a novel cdc2 + /CDC28‐related protein kinase from rice
Author(s) -
Hata Shingo
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80271-4
Subject(s) - complementary dna , cdna library , microbiology and biotechnology , cyclin dependent kinase 1 , biology , protein kinase a , peptide sequence , biochemistry , subfamily , kinase , gene , cell cycle
A cDNA clone, named R2, has been isolated by screening a rice cell cDNA library with a redundant oligonucleotide probe derived from the conserved ATP binding site of cdc2 + /CDC28 protein kinases. The cDNA contained the entire coding sequence for a 424 amino acid polypeptide with a molecular mass of 47.6 kDa. The R2 mRNA, 2.1 kb in size, was expressed in both cultured rice cells and rice seedings at similar levels. The predicted R2 protein has canonical motifs for ATP binding and catalysis, and is significantly homologous (up to 47%) to members of the cdc2 + / CDC28 subfamily of serine/threonine protein kinase. The R2 protein is a novel member of the subfamily.