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ATP‐dependent leukotriene export from mastocytoma cells
Author(s) -
Schaub Thomas,
Ishikawa Toshihisa,
Keppler Dietrich
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80256-3
Subject(s) - mastocytoma , leukotriene c4 , chemistry , leukotriene , microbiology and biotechnology , biochemistry , immunology , biology , asthma , in vitro
The biosynthesis of leukotrienes (LT) C 4 and B 4 is followed by an export of these mediators into the extracellular space. This transport was characterized using plasma membrane vesicles prepared from mastocytoma cells and identified as an ATP‐dependent primary active process. The apparent K m ‐values were 110 nM for LTC 4 and 48 μM for ATP. The transport rate was highest for LTC 4 , whereas LTD 4 , LTE 4 , and N ‐acetyl‐LTE 4 were transported with relative rates of 31, 12 and 8%, respectively, at a concentation of 10nM. LTB 4 transport was also dependent on ATP, LTC 4 transport with inhibited by LTD 4 receptor antagonists (IC 10 = 1.0 μM for MK‐571 and 1.3 μM for LY245769) and by the inhibitor of leukotriene biosynthesis MK‐886 (IC 10 = 1.8 μM). The ATP‐dependent export carrier for leukotrienes in leukotriene‐synthesizing cells represents a novel member of the family of ATP‐dependent exit pumps.